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. 2020 Mar 25;219(4):e201909178. doi: 10.1083/jcb.201909178

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© 2020 Jarsch et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S2. — SNX9 is the antigen to scFvs 3, 4, 5, and 21. (A) Extracts, control, and IgGFLS3 immunoprecipitation. (B) Venn diagram of mass spectrometry identification from the gel sectors indicated in A. (C) SNX9 gives the highest protein score and number of unique peptides. (D) Western blot of purified SNAP-SNX9 (left lane = 121 nM, right = 302.5 nM) with scFvs 3 and 4. (E) Western blot of Xenopus egg extracts and purified SNX9 for scFv5 and 21, which detect a specific ∼70 kD band (red arrow), though scFv21 does not detect purified SNX9. (F) TIRF and confocal imaging of FLS immunolabeled using a rabbit anti-SNX9 polyclonal antibody illustrating SNX9 (green) localized at FLS tips as marked by actin (magenta; TIRF in isolated channel, maximal projection of a 1 µm z-stack in merged image). Scale bar, 10 µm.