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. 2020 Apr 3;11:529. doi: 10.3389/fimmu.2020.00529

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Copyright © 2020 Casciano, Diani, Altomare, Granucci, Secchiero, Banfi and Reali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential expression of CCR4 in CD8⁺ T_CM and T_EM cells. PBMCs isolated from healthy control subjects were stained for CD8, memory T cell phenotype markers (CD45RA and CCR7) and for chemokine receptors CCR4 and CCR5. (A) CD8⁺ T cells gated as CD45RA⁻CCR7⁺ T_CM and CD45RA⁻CCR7⁻ T_EM were analyzed for the expression of CCR4 and CCR5. Representative analysis is shown in the figure. The axis scales for fluorescence are reported as log. Statistical analysis of the differences was performed by Mann–Whitney test. p-values < 0.05 were considered significant: ****p < 0.0001. (B) Mean values of the percentage of CCR4/CCR5 subpopulations among T_CM and T_EM cells were shown in pie charts.