Figure 2.

CCR4 expression characterizes the T_CM compartment. PBMCs isolated from healthy control subjects were stained for CD8, memory T cell phenotype markers (CD45RA and CCR7) and for chemokine receptors CCR4 and CCR5. (A) CD8⁺ T cells were analyzed for the memory phenotype according to CD45RA, CCR7 expression and for the expression of the chemokine receptors CCR4 and CCR5. On the basis of the chemokine receptor expression we identified five subsets CCR4^hi (gate 1), CCR4^int (gate 2), CCR4⁻CCR5⁻ (gate 3), CCR4⁻CCR5⁺ (gate 4) and CCR4⁺CCR5⁺ (gate 5). These five subsets were superimposed to the density plot of the CD45RA⁻ gated cells. Each red dot identifies cells from the corresponding subset as reported in the figure. The axis scales for fluorescence are reported as log.