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Effect of miR-146a on the Hb-induced inflammatory response and microglial polarization at different time points. Microglia were pretreated with miR-146a (50 nM) for 12 h and then Hb (20 μM) was added for 1 to 24 h. Data are presented as the fold change calculated relative to the Hb+nc group (baseline = 1). Values are means ± SD (n = 3). Statistical analysis was performed using a Mann–Whitney U-test for each gene at each time point. *p < 0.05. nc miR-146a mimic negative control.
