FIGURE 6.

Effects of miR-146a on the expression of proteins in the TLR4/TRAF6/IRAK1/NF-κB signaling pathway. (A) Western blotting results of these proteins. (B) Corresponding quantification analysis of A. Primary microglial cells were preincubated with miR-146a (50 nM) or miR-146a negative control (nc, 50 nM) for 12 h, and then Hb (20 μM) was added for 15 min. TRAF6 levels were normalized to β-actin expression, and P-p65, P-p38, P-jnk, P-p44, and P-p42 were normalized to their corresponding non-phosphorylation proteins. Values are means ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s test for multicomparison. **p < 0.01, ***p < 0.001, ##p < 0.01, and ns = no significance. nc miR-146a mimic negative control.