Table 1.
Characteristics of the four primer sets used to amplify the marker regions.
| Target name | Primer name | Primer sequence (5′–3′) | T m | Hairpin Tm | Target | Amplicon length (bp) | Haplotypes | SNPs |
|---|---|---|---|---|---|---|---|---|
| CDS-1 | GT1-F | CTCCTTGCTGCTCAGAACGA | 60 | none | ATP synthase | 175 | 2 | 7 |
| GT1-R | CAAGAGAGGAGCAGTGGCAA | 60 | 44.6 | |||||
| CDS-2 | GT2-F | TGCAAACTACTAAGGGCGCA | 60 | none | U3 small nucleolar RNA-associated protein 11 ((LOC34621252) | 246 | 2 | 1 |
| GT2N-R | CGCCTTCTCTTGAGCCTTGA | 60 | none | |||||
| CDS-3 | GT3-F | AATCGAATCGGTGCAGTGCTTA | 60.7 | none | Uncharacterized (LOC34620832) | 220 | 3 | 2 |
| GT3N-R | GACTGAACGTGTGAGAGGGG | 59.3 | none | |||||
| CDS-4 | GT4-F | GTAGATGGGTCCTTGAAGGCT | 59.2 | none | ATP-dependent RNA helicase rrp3 (LOC34619020) | 179 | 2 | 3 |
| GT4N-F | CAGACGCCTAAGGAACCGAA | 60 | 37.6 |