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. 2020 Apr 3;11:383. doi: 10.3389/fphar.2020.00383

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Copyright © 2020 Song, Zhao, Wang, Wang, Chen, Zhao, Wang, Tian, Yu and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of various concentrations of Zi Shen Huo Luo Formula (ZSHLF)-containing serum on the sizes of H9c2 cells and the proliferation of rat cardiac fibroblasts (RCFs). (A) Immunofluorescence staining of α-actinin in cardiomyocytes in different groups at different concentrations. Immunofluorescence staining was observed at 400× magnification. (B) Effects of ZSHLF-containing serum on cardiomyocytes surface area (10–15 cells per field, 5 fields per group; the data represent the mean ± standard deviation). (C) Effects of ZSHLF-containing serum on the proliferation of cardiac fibroblasts (the data represent the mean ± standard deviation, n=3). ^#P < 0.05 vs. the BC group; ^◇P < 0.05 vs. the ALD group; ^◆P < 0.05 vs. the 5% ZSHLF group.