Table 1.
Transcripts for putative starch-degrading enzymes in S. multiformis and the presence/absence of corresponding orthologs in other unicellular holozoan lineages and Metazoa
| S. multiformis | Ichthyosporea | Filasterea | Choanoflagellates | Metazoa | |
|---|---|---|---|---|---|
| α-Amylases (PF00128) | |||||
| 1 | Colp12_sorted@267, Colp12_sorted@268, Colp12_sorted@269 | Pirum_gemmata@Unigene16826_1* | Ministeria_vibrans@37663/4* | Craspedida_sp_A TCC_50635@246766* | NA |
| 2 | Colp12_sorted@19603 | NA | NA | NA | NA |
| 3 | Colp12_sorted@18987, Colp12_sorted@16467, Colp12_sorted@17178 | Pirum_gemmata@Unigene17488_1 | Ministeria_vibrans@28157 | Salpingoeca_rosetta@00083T0 | Amphimedon_queenslandica@15720304 |
| 4 = paralog to 3 | Colp12_sorted@8715 | See 3 | See 3 | See 3 | See 3 |
| 5 (SLC3A1) | Colp12_sorted@10150 | NA | NA | Salpingoeca_rosetta@08827T0 | Homo_sapiens@ENSP00000260649 |
| α-Glucosidases (PF01055) | |||||
| 1 | Colp12_sorted@9290, Colp12_sorted@22295 | Pirum_gemmata@Unigene@34248_1* | Ministeria_vibrans@34861 | Salpingoeca_rosetta@00005T0* | NA |
| 2 | Colp12_sorted@1339, Colp12_sorted@1341 | Creolimax_fragrantissima@8464T1 | Capsaspora_owczarzaki@04827T0 | NA | Amphimedon_queenslandica@15725226 |
| 3 = paralog to 2 | Colp12_sorted@9196, Colp12_sorted@14409, Colp12_sorted@18957, Colp12_sorted@21457, Colp12_sorted@31517 | See 2 | See 2 | NA | See 2 |
| 4. (GANAB/GANC) | Colp12_sorted@13767 | Creolimax_fragrantissima@4769T1 | Capsaspora_owczarzaki@00308T0 | Salpingoeca_rosetta@11360T0 | Homo_sapiens@ENSP00000326227/ENSP00000349053/ENSP00000340466 |
| Glycogen phosphorylase (PYGB/PYGL/PYGM) | |||||
| 1 | Colp12_sorted@1564 | Creolimax_fragrantissima@2598T1 | Capsaspora_owczarzaki@07395T0 | Salpingoeca_rosetta@08531T0 | Homo_sapiens@ENSP00000216392/ENSP00000216962/ENSP00000164139 |
| Glycogen debranching enzyme (AGL) | |||||
| 1 | Colp12_sorted@14615 | Creolimax_fragrantissima@4755T1 | Capsaspora_owczarzaki@06406T0 | Salpingoeca_rosetta@10237T0 | Homo_sapiens@ENSP00000355106 |
Accession numbers for S. multiformis are from the corresponding TransDecoder output files as described in Hehenberger et al. [37]; sequence identifiers for all other taxa correspond to the deposited alignments/phylogenies. Pfam domains used to identify candidates in S. multiformis and/or annotated direct human orthologous genes are indicated in brackets in column 1. NA, no direct orthologs recovered in our dataset; transcript identifiers in italic, short fragment(s) of one lineage representative
*Unclear whether sequences are direct orthologs or paralogs to S. multiformis (unresolved tree)