Figure 5.

Expression of signal-related genes in transgenic and wild-type (WT) plants treated with powdery mildew. The samples of two genetically-modified tobacco lines (L5, L8) were used to analyze genes expression by qRT-PCR. A, NtNPR1; B, NtPAL; C, NtPR1a; D, NtPR5; E, NtPDF1.2. There were four treatments: L5/L8-CK represents transgenic lines under normal conditions; L5/L8-PM represents transgenic lines infected with powdery mildew; WT-CK represents WT plants under normal conditions; WT-PM represents WT plants infected with powdery mildew. Tobacco NtEF1-α gene (AF120093) was used as an internal reference. The expression levels of signal-related genes in WT plants at 0 h were used as control (quantities of calibrator) and assumed as 1. Three biological triplicates per line were averaged and Bars indicate standard error of the mean. Data between transgenic lines and WT plants (L5/L8-PM vs. WT-PM and L5/L8-CK vs. WT-CK) were analyzed by one-way ANOVA, and *denotes statistical significance at p < 0.05.