Table 2. Identification and analysis of proteins differentially expressed in response to ETH inhibitor on H2-induced adventitious root development in cucumber explants.
| Spot No.a | Identified proteinb | Species | Scorec | NCBI accessionnumber | MWd | Pie | PeptidesCountf | Up/Downg |
|---|---|---|---|---|---|---|---|---|
| K9/1628 | Glutamine synthetase | Cucumis sativus | 406 | KGN57792.1 | 39251.8 | 5.82 | 13 | ↓ |
| K10/2129 | ribulose bisphosphate carboxylase/oxygenase precursor peptide | Cucumis sativus | 78 | AAA33131.1 | 21049.4 | 7.55 | 12 | ↑ |
| K11/1937 | Unknow protein | Cucumis sativus | 234 | KGN44928.1 | 22556.6 | 4.45 | 13 | ↓ |
| K12/1908 | Cytosolic ascorbate peroxidase | Cucumis sativus | 595 | KGN65655.1 | 27377.8 | 5.43 | 17 | ↑ |
| K13/1561 | Sedoheptulose-1,7-bisphosphatase | Cucumis sativus | 534 | KGN50632.1 | 42075.4 | 5.96 | 22 | ↑ |
| K14/1799 | Eukaryotic translation elongation factor 1 delta isoform X1 | Cucumis sativus | 417 | XP_011652060.1 | 24670.3 | 4.45 | 15 | ↓ |
| K15/1817 | Oxygen-evolving enhancer protein 1 | Cucumis sativus | 905 | KGN48464.1 | 34938.8 | 6.24 | 24 | ↓ |
| K16/2139 | PREDICTED:11S globulin seed storage protein 2-like | Cucumis sativus | 458 | XP_011652795.1 | 54382 | 5.71 | 11 | ↓ |
| K17/2062 | Cytochrome b6-f complex iron-sulfur subunit | Cucumis sativus | 112 | KGN43571.1 | 24255.2 | 8.45 | 9 | ↓ |
| K18/1169 | Chloroplast transketolase | Cucumis sativus | 712 | KGN56609.1 | 80569.6 | 6 | 27 | ↓ |
| K19/2156 | Ribulose bisphosphate carboxylase small chain | Cucumis sativus | 68 | KGN52085.1 | 20685.2 | 8.24 | 11 | ↓ |
| K20/1284 | ATPase alpha subunit (chloroplast) | Cucumis sativus | 385 | AAZ94637.1 | 55348 | 5.13 | 24 | ↑ |
| K21/2064 | ATPase alpha subunit (chloroplast) | Cucumis sativus | 246 | AAZ94637.1 | 55348 | 5.13 | 20 | ↓ |
| K22/361 | 26S protease regulatory subunit 6A homolog | Cucumis sativus | 230 | XP_004135596.1 | 38614.9 | 5.16 | 21 | ↓ |
| K23/748 | Oxygen-evolving enhancer protein 1 | Cucumis sativus | 752 | KGN48464.1 | 34938.8 | 6.24 | 20 | ↓ |
| K24/533 | Mg-protoporphyrin IX chelatase | Cucumis sativus | 236 | KGN49283.1 | 45728 | 5.72 | 20 | ↓ |
| L1/203 | Protein disulfide-isomerase | Cucumis sativus | 83 | KGN47715.1 | 57045.6 | 4.88 | 12 | ↑ |
| L2/230 | Threonine dehydratase | Cucumis sativus | 620 | KGN48214.1 | 67198.9 | 6.22 | 25 | ↑ |
| L3/1196 | PSII reaction center subunit V (chloroplast) | Cucumis sativus | 103 | AAZ94668.1 | 9380.7 | 4.83 | 4 | ↓ |
| L4/858 | Cytosolic ascorbate peroxidase | Cucumis sativus | 716 | KGN65655.1 | 27377.8 | 5.43 | 15 | ↑ |
| L6/656 | Proteasome subunit alpha type | Cucumis sativus | 466 | KGN62618.1 | 30861.4 | 4.98 | 9 | ↓ |
| L7/1009 | Unknow | Cucumis sativus | 412 | KGN57117.1 | 18646.5 | 4.56 | 9 | ↓ |
| L8/158 | Malic enzyme | Cucumis sativus | 496 | KGN66170.1 | 65093.8 | 5.72 | 23 | ↑ |
| L9/975 | Chlorophyll a-b binding protein, chloroplastic | Cucumis sativus | 270 | KGN51208.1 | 26517.6 | 5.84 | 7 | ↑ |
| L10/1116 | 60S acidic ribosomal protein P1 | Cucumis sativus | 355 | KGN56349.1 | 11414.7 | 4.34 | 3 | ↑ |
| L11/747 | Cysteine protease | Cucumis sativus | 114 | KGN62647.1 | 40614.9 | 5.2 | 8 | ↓ |
| L12/200 | Protein disulfide-isomerase | Cucumis sativus | 90 | KGN47715.1 | 57045.6 | 4.88 | 18 | ↓ |
| L13/527 | Mg-protoporphyrin IX chelatase | Cucumis sativus | 200 | KGN49283.1 | 45728 | 5.72 | 19 | ↓ |
| L14/1132 | Ribulose bisphosphate carboxylase small chain | Cucumis sativus | 100 | KGN52085.1 | 20685.2 | 8.24 | 11 | ↑ |
| L15/502 | Unknow | Cucumis sativus | 312 | KGN57401.1 | 31417.5 | 4.82 | 5 | ↓ |
| L16/626 | Sedoheptulose-1,7-bisphosphatase | Cucumis sativus | 546 | KGN50632.1 | 42075.4 | 5.96 | 18 | ↑ |
| L17/896 | PREDICTED: 29 kDa ribonucleoprotein, chloroplastic | Cucumis sativus | 298 | XP_004137828.1 | 30479.5 | 5.84 | 7 | ↓ |
| L18/103 | Chloroplast HSP70 | Cucumis sativus | 412 | KGN61439.1 | 75350 | 5.18 | 25 | ↓ |
| L19/447 | 30S ribosomal protein S1, chloroplastic | Cucumis sativus | 496 | XP_004147619.1 | 45304.5 | 5.34 | 18 | ↓ |
| L20/1129 | PREDICTED: 60S acidic ribosomal protein P2-4 | Cucumis sativus | 93 | XP_004150488.1 | 11419.9 | 4.53 | 8 | ↓ |
| L21/982 | PREDICTED: 11S globulin subunit beta | Cucumis sativus | 479 | XP_011651441.1 | 57575.8 | 7.21 | 15 | ↓ |
| L22/1140 | ribulose-1,5-bisphosphate carboxylase small subunit | Cucumis sativus | 113 | CCF55356.1 | 20713.2 | 8.24 | 15 | ↑ |
| L23/732 | PREDICTED: 14-3-3-like protein D | Cucumis sativus | 120 | XP_004136830.1 | 29379.5 | 4.76 | 10 | ↓ |
| M15/139 | PREDICTED: ruBisCO large subunit-binding protein subunit alpha | Cucumis sativus | 401 | XP_004145754.1 | 61399.9 | 5.06 | 26 | ↓ |
| M17/239 | Oxygen-evolving enhancer protein 1 | Cucumis sativus | 216 | KGN48464.1 | 34938.8 | 6.24 | 19 | |
| M18/1018 | PREDICTED: 60S acidic ribosomal protein P1 | Cucumis sativus | 170 | KGN56349.1 | 11414.7 | 4.34 | 2 | ↓ |
| M19/1030 | Cytosolic ascorbate peroxidase | Cucumis sativus | 407 | KGN65655.1 | 27377.8 | 5.43 | 17 | ↓ |
| M20/340 | ribulose-1,5-bisphosphate carboxylase small subunit | Cucumis sativus | 137 | CCF55356.1 | 20713.2. | 8.24 | 14 | ↓ |
| M21/162 | Threonine dehydratase | Cucumis sativus | 206 | KGN48214.1 | 67198.9 | 6.22 | 22 | ↓ |
| M23/462 | Sedoheptulose-1,7-bisphosphatase | Cucumis sativus | 250 | KGN50632.1 | 42075.4 | 5.96 | 18 | ↓ |
| M24/354 | Beta-form rubisco activase | Cucumis sativus | 241 | KGN50568.1 | 48292.6 | 8.19 | 19 | ↓ |
| N1/351 | Unknow | Cucumis sativus | 238 | KGN59544.1 | 36680 | 4.7 | 23 | ↓ |
| N2/117 | Protein disulfide-isomerase | Cucumis sativus | 62 | KGN47715.1 | 57045.6 | 4.88 | 16 | ↓ |
Notes.
a
Spot numbers are arbitrary as assigned by the software of PD-Quest 8.0.
b
The name and functional categories of the proteins using MALDI TOF-TOF MS.
c
The Mascot score obtained after searching against the NCBI nr database.
d,e
Theoretical molecular mass (MV) and isoelectric point (PI) of the identified protein.
f
Number of peptides sequenced.
g
“ ↑” represents up-regulated expression, “ ↓” represents down-regulated expression.
Spots K9/1628 to K21/2064 were the proteins in ETH treatment; K22/361 to L23/732 were the proteins in ETH treatment and the control; M15/139 to N2/117 were the proteins in HRW+AVG treatment