Figure 2. Kiss1^ARH Neuron Signaling is Necessary for Robust Dark-phase Locomotor Activity.

A) Waveform of daily running-wheel activity. 3-d average for each mouse. Two-way ANOVA: n = 8 (TeTx), n = 7 (YFP), F_{(1, 2002)} = 57.61, p < 0.0001 (main effect of TeTx); F_{(143, 2022)} = 27.55, p < 0.0001 (group x time interaction). Insert shows the 24-h cumulative wheel activity in the light and dark phases of the L:D cycle. Two-way ANOVA: F_{(1, 28)} = 465.7, p < 0.0001 (main effect of TeTx).

B) Double-plotted actograms of running-wheel activity from two representative mice in each cohort. Note, the activity of Kiss1^ARH-silenced mice (bottom, purple boxes) was amplified by a factor of 25X in order to visualize the pattern of wheel-running activity in each 10-min bin.

C) Double-plotted actograms of home-cage activity from two representative mice, recorded with telemetry IP transponders. The pink line indicates the day of viral injection.

D) Waveform of home-cage activity counts (3 d average for each mouse) comparing the Kiss1^Cre females before (black, Seg.1) and after viral injection (purple, Seg.3). Two-way rmANOVA: n = 6, F_{(1, 360)} = 230.7, p < 0.0001 (segment effect); F_{(71, 360}) = 3.59, p < 0.0001 (segment x time interaction). Insert shows the 24-h cumulative home-cage activity in the light and dark phases of the L:D cycle. Two-way ANOVA: F_{(1, 10)} = 21.80, p = 0.0009 (main effect of TeTx).

Error bars represent ± SEM. Grey shade indicates the dark phase of the L:D cycle. See also Figures S2 and S3