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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Curr Biol. 2019 Feb 7;29(4):592–604.e4. doi: 10.1016/j.cub.2019.01.022

Figure 3. Circadian Home-cage Activity of Kiss1ARH-silenced Mice Shows Decreased Amplitude.

Figure 3.

A) Representative actograms of home-cage activity (tracked with infrared beams) from two mice in each cohort, double plotted. Corresponding χ2 periodograms are shown below each actogram. Statistical significance (p = 0.05) is shown as a horizontal line.

B) Average period (t) of home-cage free-running locomotor activity comparing YFP-injected controls to Kiss1ARH-silenced females. Student t-test: YFP n = 7, t = 1431 ± 7.87 min; TeTx n = 5, t = 1437 ± 8.37 min, t(10) = 1.33, p = 0.21.

C) Waveform of daily activity. 5-d average was calculated based on the free-running period of each mouse. CT 12:00 was aligned with the onset of activity. We assumed the subjective night to be from CT 12:00-0:00 (12 circadian hours). Two-way ANOVA: YFP (n = 7), TeTx (n = 5); F(1, 1430) = 6.36, p = 0.03 (main effect of TeTx).

D) Cumulative 24-h activity counts, grouped into subjective day (SD) and night (SN). Student t-test comparison: YFP n = 7, t = 110.9 ± 26.45 min; TeTx n = 5, t = 77.92 ± 14.27 min, t(10) = 2.52, p = 0.030.

See also Figure S2 and S3