TABLE 3.
rDNA promoter methylation level (mean values or CpG unit) | Fertilization (2 pronuclei) rate | Good-quality embryo rate on day 3 |
Mean methylation values of all CpG units | 0.2350 (P = 0.0707) | 0.0954 (P = 0.4684) |
Mean methylation values measured using primerS1 | 0.2070 (P = 0.1126) | 0.0754 (P = 0.5670) |
Mean methylation values measured using primerS2 | 0.2528 (P = 0.0513) | 0.1002 (P = 0.4461) |
Mean methylation values measured using primerS3 | 0.1857 (P = 0.1555) | 0.0874 (P = 0.5068) |
CpG_1 | 0.2274 (P = 0.0806) | 0.0446 (P = 0.7354) |
CpG_2 | 0.0336 (P = 0.7987) | 0.0988 (P = 0.4526) |
CpG_3 | 0.2371 (P = 0.0682) | 0.0649 (P = 0.6223) |
CpG_4 | 0.2316 (P = 0.0749) | 0.0867 (P = 0.5100) |
CpG_5 | 0.1108 (P = 0.3995) | 0.0852 (P = 0.5176) |
CpG_6 | 0.2419 (P = 0.0626) | 0.1042 (P = 0.4283) |
CpG_7 | 0.2512 (P = 0.0529) | 0.1354 (P = 0.3022) |
CpG_8 | 0.2459 (P = 0.0582) | 0.0763 (P = 0.5623) |
CpG_9 | 0.2010 (P = 0.1235) | 0.1026 (P = 0.4354) |
CpG_10 | 0.3055 (P = 0.0176)* | 0.1383 (P = 0.2920) |
CpG_11 | 0.2613 (P = 0.0438)* | 0.0475 (P = 0.7185) |
CpG_12 | 0.2049 (P = 0.1163) | 0.1024 (P = 0.4360) |
CpG_13 | 0.2032 (P = 0.1194) | 0.0790 (P = 0.5484) |
CpG_14 | 0.2928 (P = 0.0232)* | 0.1041 (P = 0.4287) |
CpG_15 | 0.1988 (P = 0.1278) | 0.0945 (P = 0.4727) |
CpG_16 | 0.1412 (P = 0.2820) | 0.0450 (P = 0.7329) |
CpG_17 | 0.1866 (P = 0.1534) | 0.0894 (P = 0.4971) |
CpG_18 | 0.1515 (P = 0.2479) | 0.0854 (P = 0.5166) |
CpG_19 | 0.1891 (P = 0.1480) | 0.0738 (P = 0.5750) |
CpG_20 | 0.2147 (P = 0.0995) | 0.0947 (P = 0.4716) |
CpG_21 | 0.1951 (P = 0.1352) | 0.0848 (P = 0.5194) |
*P < 0.05.