Figure 6.

Homeostasis and cytokine expression of NCOR1-deficient CD4⁺ T cells upon transfer. (A) Naïve WT or NCOR1 cKO^Cd4 CD4⁺ T cells were i.p. injected into Rag2^−/− mice and 8 weeks later transferred CD4⁺ T cells in recipient mice were analyzed. Contour plots show TCRβ expression vs. side scatter (SSC-A) of cells isolated from spleen, mLNs, small intestine IELs (SI-IELs) and lamina propria cells (SI-LPs) of recipient Rag2^−/− mice. (B) Diagrams depict the percentages of TCRβ⁺ cells (upper panel) and numbers of TCRβ⁺ CD4⁺ cells (lower panel) in the indicated organs of Rag2^−/− mice that have received either naïve WT or NCOR1 cKO^Cd4 CD4⁺ T cells. (C) Contour plots depict IL-17A vs. IFNγ expression of cells isolated from spleen, mLNs, SI-IELs, and SI-LP cells of recipient Rag2^−/− mice. (D) Diagrams indicating percentages of IFNγ single-producing cells (IFNγ⁺IL-17A⁻) (upper panel), IL-17A single-producing cells (IFNγ⁻IL-17A⁺) (middle panel) and IFNγ⁺IL-17A⁺ double-producing (lower panel) TCRβ⁺ cells isolated from Rag2^−/− mice that have received either naïve WT or NCOR1 cKO^Cd4 CD4⁺ T cells. (A,C) Representative plots for the various organs and tissues were taken from different experiments, however for each organ/tissue shown WT and NCOR1 cKO^Cd4 plots are from the same experiment. Numbers indicate the percentage of cells in the respective quadrants or regions. (B,D) Horizontal bars indicate the mean. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant (unpaired two-tailed t-test). Data are representative (A,C) or show summary (B,D) of 6–8 mice that were analyzed in 3 independent experiments.