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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1389–1400. doi: 10.1167/iovs.06-0677

FIGURE 3.

FIGURE 3.

Influence of CNTF on bipolar cells in the rds retina. Immunofluorescent images of adult wild-type (WT) retinas (A, D), P90 (B, C, E, F) and P72 (H, I) rds retinas treated with rAAV-CNTF at P23 (C, F, I) and the untreated rds controls (B, E, H) labeled for 115A10 (A–C; A′–C′: higher magnifications of A–C), Chx10 and CycD3 (D–F), and Chx10 (H, I) are shown. (H, I) Examples of the confocal optical sections used for the quantification of Chx10 in (G). (A′–C′, white arrows) Cell bodies labeled by 115A10. (I, white arrowheads) Ectopic Chx10-positive cells in the ONL. (G) Quantification of Chx10-positive cells at P70 (n = 4, 16 fields each) per unit length (230 μm) of retina (1.7-μm-thick confocal optical sections). *Statistically significant sample pairs: P < 0.001 between untreated and CNTF-treated rds retinas, and between wild-type (WT) and CNTF-treated rds retinas. onl, outer nuclear layer; opl, outer plexiform layer; inl, inner nuclear layer; ipl, inner plexiform layer; gcl, ganglion cell layer. Scale bars: (A–C) 100 μm; (D–F, H, I) 50 μm; (A′–C′) magnification of (A–C) ×3.