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Effects of rAAV-CNTF treatment on retinal cell death. Immunofluorescent images of P90 rds retinas treated with rAAV-CNTF at P23 (C, D) and untreated control retinas (A, B) labeled by TUNEL (A, C) and 4′,6′-diamino-2-phenylindole (DAPI; B, D) are shown. (A, C, arrowheads) Cells undergoing apoptosis. Increased apoptosis was detected in the ganglion cell layers of the treated P90 rds retinas (C). Abbreviations as in Figure 3. Scale bar, 100 μm.
