Table 2. Disease scoring of bean roots from disease-suppressive assay.
Disease Indices† | ||
---|---|---|
Root rot index‡ | Necrotic lesion index§ | |
Autoclaved soil | ||
Bean | 0.005 ± 0.004a | 0.258 ± 0.163a |
Bean + Fusarium | 0.225 ± 0.117b | 1.600 ± 0.414b |
Bean + Fusarium + Metarhizium | 0.040 ± 0.039a | 0.450 ± 0.376a |
Microbiome soil | ||
Bean | 0.014 ± 0.011a | 0.175 ± 0.065a |
Bean + Fusarium | 0.020 ± 0.014a | 0.155 ± 0.042a |
Bean + Fusarium + Metarhizium | 0.020 ± 0.008a | 0.160 ± 0.047a |
† Plants were treated with Fusarium solani f. sp. phaseoli with or without Metarhizium robertsii and grown in microbiome soil or autoclaved soil for 14 days. The average of each disease metric ± the standard deviation, is shown. Values followed by the same letter are not significantly different (Scheffe’s post hoc, α = 0.05; n = 3)
‡Dar et al. (1997) root rot disease index scored from 0 = no disease, to 1.00 = greater than 75% root area rotted.
§Filion et al. (2003) necrotic lesions scored from 0–5; 0 = no disease symptoms; 1 = slightly brown or <50% surface discoloration of the hypocotyl, firm upon pressure from thumb and forefinger, and slight root pruning; 2 = as 1 but >50% surface discoloration; 3 = discolored hypocotyl and roots collapsing under considerable pressure and extensive root pruning; 4 = darkly discolored hypocotyl and roots completely collapsed or collapsing easily under pressure and severe root pruning; 5 = dead or dying plant.