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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 21;34(4):5563–5577. doi: 10.1096/fj.201903134R

Figure 3:

Figure 3:

Effects of Phenylephrine (PE) on membrane potentials recorded from circular SMCs of mouse distal colon. Intracellular recordings were performed in the presence of TTX (1 μM) to inhibit neural activity. A: PE (1 and 10 μM) induced a hyperpolarization. B: PE-induced hyperpolarization was abolished by pretreatment of RS100329 (100nM). C: Apamin (Apa) 300nM inhibited PE-induced hyperpolarization. The resting membrane potentials were: A: −47 mV; B: −42 mV; C: −46 mV. A-C were recorded from different tissues. Each record in a given set of two was obtained from the same impalement. D: Summary showing the effects of RS100329 and Apa on PE-induced hyperpolarization. * P < 0.0001, significant difference from ΔV of PE (1 μM).†P < 0.0001, significant difference from ΔV of PE (10 μM).