. Author manuscript; available in PMC: 2020 Sep 4.

Published in final edited form as: Nature. 2020 Mar 4;579(7799):427–432. doi: 10.1038/s41586-020-2078-2

Extended Data Table 2.

Protospacer sequences of individually cloned sgRNAs

Target gene	sgRNA	Protospacer (5’ to 3’)
HRI	sgRNA1	GTAGCTGCAGCATCGGAGTG
HRI	sgRNA2	GACGGCGCTAGCTGCAGCAT
PKR		GGCGGCGGCGCAGGTGAGCA
GCN2		GCAGCGCTGCGCCCAAGGCA
PERK		GCAGAGGCCGGGCTGAGACG
DELE1	sgRNA1	GAGACCAACCCTTGGGACAG
DELE1	sgRNA2	GGCCCGCCCCCACTCCAGTT
OMA1	sgRNA1	GCGAGTAGGATCGTGCCCAG
	sgRNA2	GCACTTCCTGCCCGCCATAC
OPA1	sgRNA1	GGAGACGGGCCAAGATTGAG
PMPCB	sgRNA1	GCCCTGCCCGACCCACTGTT
PMPCB	sgRNA2	GCGCCACAGATCCGAACAGT
HSPD1		GTTCGGAAAGAAGGACACGT
MRPL17		GGCCCATACGGCGAAATACG
MRPL22		GGTAGCGGGAGGGCGAAAGA
LONP1		GGGGCTATGGCGGCGAGCAC
Non-targeting control (NTC) sgRNAs	1872	GTCCACCCTTATCTAGGCTA
	2151	GTGCCAGCTTGTGGTGTCGT
	2152	GCGACATCGGTTACATGTGG
	2153	GTAGACGTGCAAAAGCCGCA