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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Eur Respir J. 2020 Mar 20;55(3):1901313. doi: 10.1183/13993003.01313-2019

Figure 2.

Figure 2.

Apoptosis assessed by TUNEL assay following treatment with vehicle and TNF-α. Panel A) Representative image of PAECs exposed to tert-butyl hydroperoxide for 6 hours TUNEL stained for apoptosis and counterstained with 4’,6-diamidino-2-phenylindole (DAPI). TUNEL positive cells are indicated by yellow arrow. Image at 20X magnification. Scale bar = 50 μm. Panel B) PAECs were exposed to vehicle or TNF-α for six hours. Data are presented as mean ± SD. n = 2 – 5; each n is derived from a different passage of cells from a single balloon tipped catheter from one right heart catheterization procedure for a single subject. *p < 0.05. X axis: No PAH = subject 14; idiopathic PAH = subject 18; HIV APAH = subject 13; PoPH, high CO = subject 7. PAEC = pulmonary artery endothelial cell; PAH = pulmonary arterial hypertension; PoPH = portopulmonary hypertension; CO = cardiac output.