Table 1.
Subject and procedural characteristics by pulmonary artery endothelial cell culture success
| Total Sample | Success | No Success | P value | |
|---|---|---|---|---|
| Balloon tips, n (%) | 49 | 19 (39) | 30 (61) | |
| Subject-level characteristics | ||||
| Age, yr | 62 (53 – 71) | 69 (54 – 78) | 61 (52 – 67) | 0.16 |
| Male sex, n (%) | 17 (35) | 8 (42) | 9 (30) | 0.29 |
| Race, n (%) | 0.86 | |||
| White | 34 (69) | 13 (68) | 21 (70) | |
| Black | 11 (22) | 4 (21) | 7 (23) | |
| Other | 3 (6) | 2 (11) | 1 (3) | |
| Body mass index, kg/m2 | 30 (25 – 33) | 32 (27 – 34) | 29 (24 – 33) | 0.25 |
| Clinical PAH diagnosis | 22 (45) | 8 (42) | 14 (47) | 0.49 |
| PAH treatment, n (%) | 19 (39) | 6 (32) | 13 (43) | 0.30 |
| Six-minute walk distance, m | 365 (182 – 425) | 355 (122 – 410) | 375 (295 – 462) | 0.09 |
| Functional class III/IV, n (%) | 15 (47) | 6 (46) | 9 (47) | 1.00 |
| No. available | 32 | 13 | 19 | |
| Brain natriuretic peptide, pg/mL | 80 (23 – 390) | 76 (32 – 413) | 80 (22 – 184) | 0.71 |
| No. available | 42 | 26 | 16 | |
| Hemodynamic PH, n (%) | 39 (80) | 16 (84) | 23 (77) | 0.40 |
| Hemodynamic PAH, n (%) | 19 (39) | 10 (53) | 9 (30) | 0.10 |
| Hemodynamics | ||||
| RAP, mm Hg | 9 (7 – 12) | 10(7 – 14) | 9 (6 – 11) | 0.40 |
| mPAP, mm Hg | 37 (25 – 46) | 38 (26 – 48) | 36 (25 – 42) | 0.34 |
| PCWP, mm Hg | 12 (10 – 16) | 13 (9 – 15) | 12 (10 – 16) | 0.77 |
| CO, L/min | 5.5 (4.4 – 7.7) | 4.7 (4.0 – 5.6) | 6.4 (4.7 – 8.1) | 0.04 |
| CI, L/min/m2 | 2.3 (2.1 – 3.5) | 2.3 (2.1 – 3.5) | 3.2 (2.8 – 4.0) | 0.03 |
| PVR, Wood units | 3.6 (1.9 – 6.5) | 5.1 (2.2 – 8.3) | 2.4 (1.5 – 6.2) | 0.04 |
| Procedural-level characteristics | ||||
| Provocative testing*, n (%) | 33 (67) | 14 (74) | 19 (63) | 0.64 |
| Most experienced operator, n (%) | 19 (39) | 7 (37) | 12 (40) | 0.77 |
| Morning collection†, n (%) | 33 (67) | 14 (74) | 19 (63) | 0.54 |
| Transit time‡, minutes | 28 (20 – 40) | 28 (25 – 40) | 28 (19 – 49) | 0.77 |
| Transport conditions, n (%) | ||||
| Media 37°C, no heater, n (%) | 43 (88) | 15 (35) | 28 (65) | 0.19 |
| Media 37°C, heater 37°C, n (%) | 6 (12) | 4 (67) | 2 (33) | |
Data represented as n (%) or median (IQR). Wilcoxon-Mann-Whitney tests were used to compare continuous variables and chi-square or Fisher’s exact tests were used to compare categorical variables, as appropriate. hemodynamic values taken from baseline assessment. All catheterizations were performed from the internal jugular position.
Maneuver such as vasoreactivity or exercise testing requiring >1 “wedging” of the catheter.
Pulmonary artery catheter tip collected before 12 pm.
Ex vivo to culture dish. PH=pulmonary hypertension, defined as mean pulmonary artery pressure (mPAP) ≥ 25 mmHg; PAH=pulmonary arterial hypertension, defined as mPAP ≥ 25 mmHg, pulmonary capillary wedge pressure (PCWP) ≤ 15 mmHg and pulmonary vascular resistance (PVR) > 3 Wood units. RAP=right atrial pressure; CO=cardiac output; CI=cardiac index.