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. 2020 Apr 10;6(15):eaay0076. doi: 10.1126/sciadv.aay0076

Fig. 4. Surfactant treatment reduces silicone surface stresses and attenuates rigidity responses.

Fig. 4

(A) Relationships between cell spread area of MECs and the elastic moduli of PA or silicone gels; n > 40 cells per condition. Experiments were conducted in triplicate. (B) Confocal fluorescence images showing the indentation profiles of silicone gels with or without pretreatment with surfactant (0.1% Span 85). Indentation measurements were made using 254-μm radius steel balls after culture of cells for 24 hours. Horizontal scale bar, 100 μm; vertical scale bar, 30 μm. (C) Percentage of dead cells among total cells growing on silicone gels versus surfactant (0.1% Span85)–pretreated silicone gels 24 hours after seeding. t test was used. A total of 190 to 280 cells were analyzed for each condition. Experiments were conducted in triplicate. (D) Quantification of cell area on silicone gels versus surfactant (0.1% Span85)–pretreated silicone gels. ***P < 0.001, t test; n ≥ 40. Experiments were conducted in triplicate. Error bars show SEMs.