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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Cell Rep. 2020 Feb 18;30(7):2065–2074.e4. doi: 10.1016/j.celrep.2020.01.073

Figure 4. miR-9–5p Supports the Metabolic Activity of GBM Stem Cells and May Influence Resistance to Therapeutic Intervention on Gliomas.

Figure 4.

(A) GBM8 neurosphere cultures: transfected with 50 nM lipofectamine plus no oligonucleotide (i, mock); FAM-labeled scrambled oligonucleotide (ii, v, scrambled); or FAM-labeled antagomir (iii, vi, miR-9–5p antisense); non-transfected GBM8 (iv). Micrographs taken 12 days after transfection (seeding at1 × 104 cells/ well). Scale bar, 200 μm.

(B) Metabolic activity (mean ± SD) of transfected cells measured by WST-1reduction assay.

(C) miR-9–5p levels (mean ± SD) in the cancer cell fraction of LGG tumors (astrocytomas or oligoastrocytomas), stratified by response to therapeutic intervention.