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. 2020 Apr 9;202(9):e00746-19. doi: 10.1128/JB.00746-19

TABLE 1.

Strains and plasmids

Plasmid Strain (reference) Characteristics
ΔMSMEG_2952 strain (71) mc2155 MSMEG_2952::Hygr
pSS303 SS-M_0486 pmyc1tetO promoter + pmyc1 5′ UTR + yfp-6×His
pSS309 SS-M_0489 pmyc1tetO promoter + sigA 5′ UTR + first 54 nt of sigA + yfp-6×His
pSS310 SS-M_0493 pmyc1tetO promoter + no 5′ UTR + yfp-6×His
pSS314 SS-M_0497 pmyc1tetO promoter with a deletion of nt −53 through −1 + yfp-6×His
pSS316 SS-M_0521 pmyc1tetO promoter + Δ1GTG sigA + first 54 nt of sigA + yfp-6×His
pSS335 SS-M_0524 pmyc1tetO promoter + Δ2GTG sigA + first 54 nt of sigA + yfp-6×His
pSS359 SS-M_0623 pmyc1tetO promoter + pmyc1 5′ UTR + first 54 nt of sigA + yfp-6×His
pSS360 SS-M_0626 pmyc1tetO promoter + no 5′ UTR + first 54 nt of sigA + yfp-6×His
pSS365 SS-M_0629 pmyc1tetO promoter with a deletion of nt −53 through −1 + first 54 nt of sigA + yfp-6×His
pSS384 SS-M_0636 pmyc1tetO promoter with a deletion of nt −53 through −1 + sigA 5′ UTR + first 54 nt of sigA + yfp-6×His
pSS385 SS-M_0639 pmyc1tetO promoter with a deletion of nt −53 through −1 + pmyc1 5′ UTR + first 54 nt of sigA + yfp-6×His