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Regulation of the grp genes of E. coli CFT073. RT-qPCR was used to measure the relative fold induction of each grp gene by 1,2-PD in wild-type CFT073 E. coli and in mutants where grpP or grpQ was deleted. Results shown are the average from three replicates, with induction calculated using the threshold cycle (ΔΔCT) method with the gapA gene as the standard. The error bars represent one standard deviation. The growth conditions and RT-qPCR methods are described in Materials and Methods.
