TABLE 1.
Growth of grp deletion mutants on 1,2-PD
| Strain or deletion mutant description | Closest homolog of deleted gene product | 1,2-PD utilizationb | Protein product of deleted gene (GenPept accession no.) |
|---|---|---|---|
| WTa | +++ | ||
| ΔgrpA | Alcohol dehydrogenase | +−− | WP_000135343.1 |
| ΔgrpB | Phosphotransacylase | +−− | WP_001109982.1 |
| ΔgrpC | EutJ-like chaperone of unknown function | ++− | WP_001332070.1 |
| ΔgrpD | Hypothetical flavoprotein | +++ | WP_001329683.1 |
| ΔgrpE | EutN MCP vertex protein | − | WP_000280292.1 |
| ΔgrpF | Heme-binding protein of unknown function | +++ | WP_001301167.1 |
| ΔgrpG | Propionate kinase | ++− | WP_001362987.1 |
| ΔgrpH | Shell hexamer | − | WP_001301171.1 |
| ΔgrpI | Shell trimer (stacked/gated) | − | WP_001205499.1 |
| ΔgrpJ | Aldehyde dehydrogenase | − | WP_000997839.1 |
| ΔgrpK | Shell hexamer with FeS cluster | +/− | WP_000746009.1 |
| ΔgrpL | Putative protease of unknown function | +/− | WP_000722279.1 |
| ΔgrpM | Glycyl radical diol dehydratase (GR-DDH) | − | WP_000890295.1 |
| ΔgrpN | grpM activating enzyme | − | WP_001044978.1 |
| ΔgrpO | Permease | +++ | WP_001086421.1 |
| ΔmtrA | Maturase-reverse transcriptase | +++ | AAN82974.1 |
| ΔmtrB | Maturase-reverse transcriptase | +++ | WP_000237741.1 |
| ΔgrpP | Histidine kinase | − | AAN82979.1 |
| ΔgrpQ | DNA-binding response regulator | − | WP_000494233.1 |
| ΔgrpR | Methionine adenosyltransferase | +++ | WP_096489982.1 |
| ΔgrpS | Shell hexamer with C-terminal extension | ++− | WP_000015571.1 |
| ΔmrtAB | Maturase-reverse transcriptase | +++ |
a
WT, wild-type E. coli CFT073.
b
Qualitative estimate of growth based on the growth curves shown in Fig. S1. A minus sign indicates no growth. One, two, or three plus signs indicate slow, medium, or normal growth, respectively.