FIG 3.

MbtS is degraded by FtsH. (A) Degradation of MbtS by membranes containing FtsH. Membranes containing MbtS were mixed with membranes containing FtsH (+ FtsH) or reaction buffer (− FtsH) in the presence of nonionic detergents. The degradation of MbtS was monitored by Western blotting. Sortase A (SrtA), a non-FtsH substrate located in the membrane, was used as a negative control. (B and C) Degradation of MbtS by purified FtsH. Strep-tagged FtsH was purified from E. coli and mixed with membranes (B) or cell lysate (C) containing MbtS. The degradation of MbtS was monitored by Western blotting. The original blots are provided in Fig. S2 in the supplemental material. The thin lines indicate the splicing point.