FIG 5.

MbtS binds the DNA containing the inverted repeat sequence. (A) Biolayer interferometry (BLI) analysis with a DNA probe containing the inverted repeat sequence and solubilized membranes purified either from the ftsH deletion mutant of USA300 (ΔftsH) or from the ΔftsH mutant with a transposon insertion in mbtS (ΔftsH::mbtS). (B) BLI analysis with membranes containing MbtS and six DNA probes (1 wild-type [WT] and 5 mutants [m1 to m5]). The mutated nucleotides in the mutant probes are shown in Fig. 4D. (C) DNA affinity chromatography to examine MbtS binding to the WT and mutant DNA probes. WT, WT strain RN4220; Δ, RN4220 with a transposon insertion in ftsH; −, no DNA probe; wt, wild-type DNA probe; m1 to m5; mutant DNA probes.