TABLE 2.
Oligonucleotides used in this study
| Category and name | Sequence (5′→3′)a | Target |
|---|---|---|
| pOS1-PmbtS-lacZ construction | ||
| P3302 | GTTCACCACCTTTTCCCTATGAATAACCATCTCGTTCCCTATACTTTTTAATTTGT | PmbtS |
| P3303 | AGCCTTAAAGACGATCCGGGAAAAAGATTAGAAAAGCATAGTTGGAAAGCT | PmbtS |
| P3304 | AGGGAACGAGATGGTTATTCATAGGGAAAAGGTGGTGAACTACTGT | lacZ |
| P3305 | GCGGGCAGTGAGCGCAACGCACGCCAAGCTTGCATGC | lacZ |
| P3306 | CAGGCATGCAAGCTTGGCGTGCGTTGCGCTCACTGC | pOS1 |
| P3307 | TATGCTTTTCTAATCTTTTTCCCGGATCGTCTTTAAGGCT | pOS1 |
| pOS1-PmbtS-lacZ deletion mutagenesis | ||
| P3330 | GCATCATTAATAAGTGAAATTCAAGTTGGCATTG | PmbtS Δ1 |
| P3331 | AGGTCTGTCTTAAGGGAGTCTTCGAAC | PmbtS Δ2 |
| P3332 | ATCATAAAGGTGACTTGATTTAACTTTATCTGATAG | PmbtS Δ3 |
| P3372 | GTCAAAACATTTAGTCAATCATAAAGG | PmbtS Δ4 |
| P3463 | GTTGTCGTATGTCAAAACATTTAGTCAATC | PmbtS Δ5 |
| pOS1-PmbtS-lacZ Δ5 point mutagenesis | ||
| P3481 | CGACAACTATGCTTTTCTAATCTTTTTCCCGG | PmbtS Δ5 |
| P3482 | TAAGTCAAAACATTTAGTCAATCATAAAGG | PmbtS Δ5 |
| P3483 | TTTGTCAAAACATTTAGTCAATCATAAAGG | PmbtS Δ5 |
| P3484 | AATGTCAAAACATTTAGTCAATCATAAAGG | PmbtS Δ5 |
| P3466 | ATACGACAACTATGCTTTTCTAATCTTTTTCCC | PmbtS Δ5 |
| P3431 | CTCAAAACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3432 | GACAAAACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3433 | GTGAAAACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3434 | GTCTAAACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3435 | GTCATAACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3436 | GTCAATACATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3437 | GTCAAATCATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3438 | GTCAAAAGATTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3439 | GTCAAAACTTTTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3440 | GTCAAAACAATTAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3441 | GTCAAAACATATAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3442 | GTCAAAACATTAAGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3443 | GTCAAAACATTTTGTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3444 | GTCAAAACATTTACTCAATCATAAAGGTGAC | PmbtS Δ5 |
| P3445 | GTCAAAACATTTAGACAATCATAAAGGTGAC | PmbtS Δ5 |
| P3446 | GTCAAAACATTTAGTGAATCATAAAGGTGAC | PmbtS Δ5 |
| P3447 | GTCAAAACATTTAGTCTATCATAAAGGTGAC | PmbtS Δ5 |
| BLI assay | ||
| P3556 | Biotin-AGGTCTGTCTTAAGGGAGTCTTCGAAC | PmbtS |
| P3557 | CAGATAAAGTTAAATCAAGTCACCTTTATG | PmbtS |
| P3670 | CAGATAAAGTTAAATCAAGTCACCTTTATGATTGACTAAATGTTTTGAGATACGACAAC | PmbtS m1 |
| P3671 | CAGATAAAGTTAAATCAAGTCACCTTTATGATTGACTAAATCTTTTGACATACGACAAC | PmbtS m2 |
| P3672 | CAGATAAAGTTAAATCAAGTCACCTTTATGATTGACAAAATGTTTTGACATACGACAAC | PmbtS m3 |
| P3673 | CAGATAAAGTTAAATCAAGTCACCTTTATGATTGTCTAAATGTTTTGACATACGACAAC | PmbtS m4 |
| P3674 | CAGATAAAGTTAAATCAAGTCACCTTTATGATTCACTAAATGTTTTGACATACGACAAC | PmbtS m5 |
| P3696 | Biotin-CTTTATCTAATCCACTGCGTCTAATTATTCCG | P2637 |
| P3697 | GGTTAGCATCGTATGTACCACTTCTTG | P2637 |
| pYJ-ftsH-His6 construction | ||
| P3764 | TAAAATAAGCTTGATATCGATGGGAAGTAGGAGGAAATG | ftsH |
| P3765 | TGACATTAGAAAACCGACTGCGTACTTCCAATCCAATGC | ftsH |
| P3766 | AGCATTGGATTGGAAGTACGCAGTCGGTTTTCTAATGTCAC | pYJ335 |
| P3767 | TCATTTCCTCCTACTTCCCATCGATATCAAGC | pYJ335 |
| pET28a-ftsH-Strep construction | ||
| P4218 | CTTTAAGAAGGAGATATACCATGCAGAAAGCTTTTCGCAATGTG | ftsH |
| P4219 | CGGATCTCATTTTTCGAACTGCGGGTGGCTCCATTTATTGTCTGGGTGATTTGGATC | ftsH |
| P4220 | TAAATGGAGCCACCCGCAGTTCGAAAAATGAGATCCGGCTGCTAACAAAGCCC | pET28a |
| P4221 | TTGCGAAAAGCTTTCTGCATGGTATATCTCCTTCTTAAAGTTAAACA | pET28a |
| qRT-PCR | ||
| P3081 | GATGATTTAGTAAAAGGGACC | mbtS |
| P3082 | GATTGCCACCGTTACACCCC | mbtS |
| P1395 | GGCGGCCTTATTGGTGCAAATG | hla |
| P1396 | CCATATACCGGGTTCCAAGA | hla |
| P2902 | CCAAGGGAACTCGTTTTACG | saeR |
| P2903 | ACGCATAGGGACTTCGTGAC | saeR |
| P3093 | GTATGGCGAAGCACAGTGATC | 2637 |
| P3094 | GGTTTAGTGGAGCCATTTATTG | 2637 |
| P3099 | CAAATGATCACAGCATTTGGTACAG | gyrB |
| P3100 | CGGCATCAGTCATAATGACGAT | gyrB |
| P4710 | TGCACCAAGAAGAACAGTGG | qcvT |
| P4711 | CGCTTTTAATTGACGCTTACG | qcvT |
| P4716 | CATTCAATTGCCAAAGCAGA | splA |
| P4717 | TTTCCTCCGCCTTTACCTTT | splA |
| P4718 | CATGCAGAAGCTGTGATGAGA | 1890 |
| P4719 | GCGTGCATACCATTTCTTGA | 1890 |
| P4720 | TGGTGATAAAGTCGCTGTGC | 2641 |
| P4721 | CGATTTTCCAGCTTTCCAAC | 2641 |
| P4727 | GCTGGTACAGGTGCTGGTTT | pstS |
| P4728 | CAACCGTTACACCATCTTGC | pstS |
| P4731 | CGGCACTACTGCTGACAAAA | spa |
| P4732 | AACGCTGCACCTAAGGCTAA | spa |
| P4733 | AGCGTTTGTCACAACTGTCAAT | 0929 |
| P4734 | ACCGAGAAATGCCTCCAAAT | 0929 |
| P4739 | GGTCATGATGATGGTGGACA | clpC |
| P4740 | ACTTGAACCACCGAATCCAG | clpC |
| P4741 | CAGTTTATGGCGCAAGAGGT | cap5A |
| P4742 | GCGAAGCTATTCGCAATTTT | cap5A |
a
Mutated nucleotides are underlined.