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Accumulation of staphylopine intermediates are toxic to S. aureus. S. aureus ΔcntKLM (A) and ΔcntKLM ΔcntE (B) mutants containing the indicated plasmids were grown in increasing concentrations of anhydrotetracycline (Atc). Growth was assessed by measuring the OD600 at 8 h. *, P < 0.05 compared to vector control at the same Atc concentration via two-way ANOVA using Dunnett’s posttest. n ≥ 3. Error bars in all panels are standard errors of the mean.
