FIG 3.
Transposon mutant strains carrying sigB operon mutations all have decreased σB activity. Stationary-phase cultures of WT, ΔsigB, and transposon mutant strains transformed with pKSV7-Plmo2230::egfp were grown at 37°C. (A and B) Measurements of fluorescence were made using images obtained by fluorescence microscopy (A) and particle quantification from fluorescence microscopy images (B). A total of 15 images were taken across three biological replicates. Particle counts were normalized against the WT strain and converted to percentages. (C) Western blotting using anti-GFP antibodies. The arrow shows the eGFP protein with a size of 27 kDa. (D) Quantification of the percentage of eGFP normalized against the WT, obtained from Western blot images. Data were generated from three independent biological replicates. Statistical analysis was performed by a paired Student t test (***, P value of <0.001; NS, nonsignificant).