Loss of σB activity is associated with increased growth rates at elevated temperatures. (A to D) Measurements of OD600 values for the WT, ΔsigB, 2RsbU (E103K), and B14:A6 strains were performed every hour for 12 h in BHI medium at 30°C (A), 37°C (B), 40°C (C), and 42°C (D). (E) Growth rates in hours were calculated by using the log10 (OD600) value for the transition period from data points between 4 and 5 h (for 37°C, 40°C, and 42°C) and between 5 and 6 h (30°C). Data were generated from three biological replicates. Statistical analysis was performed using a paired Student t test (*, P value of <0.05; **, P value of <0.01; ***, P value of <0.001; NS, nonsignificant).