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. 2020 Apr 9;202(9):e00751-19. doi: 10.1128/JB.00751-19

TABLE 3.

Primers used for this study

Primer sequence (5′–3′) Target
GCCTTGTCGCCATCTTTG egfp-lmo2230-F integration
GGCCGTTTACATCTCCATC egfp-lmo2230-R integration
ATAACGGCACAAGCTTCG sigB-flank_F
TTATGGCGTCAACAGTCG sigB-flank_R
GGAGTAAATGAACAAGGCAG rsbX-flank_F
CGCTAGTTTAAAAGGTGTTATGG rsbX-flank_R
CGGTCGACGTAGAGTCCATCGCCCGAA rsbX-A
TTACTCCACTTCCTCATTCTGCAAC rsbX-B
AATGAGGAAGTGGAGTAACCATAACACC rsbX-C
CGAGATCTATCATTCCGGCAACAAGTAAATCTTGG rsbX-D
CTAGCTAATGTTACGTTACA pMAD-U
GCGAGAAGAATCATAATGGG pMAD-L
GAAAATAAATTCCGGTTGCTAAGGC lmo0596-A
AATCGAGTCGGATGGTTCTTGTT lmo0596-B
CCACTCCTCTTTGATATGTATTTAT lmo0596-flank_F
GGCAGATGAATGCACTTATG lmo0596-flank_R
RT-qPCR
    TGGGGAGCAAACAGGATTAG 16S_F
    TAAGGTTCTTCGCGTTGCTT 16S_R
    CATATTCGAAGTGCCATTGC lmo2230_F
    CTGAACTAGGTGAATAAGACAAAC lmo2230_R
    CCTAAACTTGGATTTCCGACTTATCTT sreB_F
    TTCTTATCACGAAAGGTGGAGGG sreB_R
    GGGTACTAGCTGACGGAATTTTATC lmo0596_F
    CCCACATACCGAAAAGTAATACGAG lmo0596_R