KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| V. parahaemolyticus VP882 lysogen | BCRC | BCRC 80155 |
| E. coli T7Express lysY/Iq | NEB | Cat# C3013I |
| E. coli TOP10 | Invitrogen | Cat# C404010 |
| E. coli cI857 lysogen | (Sussman and Jacob, 1962); gift of Tom Silhavy | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| SNAP-JF503 | Lavis Lab | N/A |
| SNAP-Cell 647-SiR | NEB | Cat# S9102S |
| HALO-TMR | Promega | Cat# G8251 |
| HALO-Alexa660 | Promega | Cat# G8471 |
| RecA and RecA Buffer | NEB | Cat# M0249L |
| ATP-γ-S | Sigma | Cat# A1388–5MG |
| Novex 6% DNA Retardation Gel | Thermo | Cat# EC63655BOX |
| SYBR Green I Nucleic Acid Gel Stain | Thermo | Cat# S7567 |
| 4–20% Mini-PROTEAN TGX Protein Gels | Bio-Rad | Cat# 4561096 |
| GeneMorph II EZClone Domain Mutagenesis Kit | Agilent | Cat# 200552 |
| Anhydrotetracycline | Takara | Cat# 631310 |
| Isopropyl β-D-1-thiogalactopyranoside | Fisher Scientific | Cat# BP1755–10 |
| Q5 High Fidelity Polymerase | NEB | Cat# M0491L |
| HiFi DNA assembly Mix | NEB | Cat# E2621L |
| Ni-NTA Superflow Resin | Qiagen | Cat# 30430 |
| Critical Commercial Assays | ||
| Synergy Neo2 Multi-Mode Reader | BioTek | N/A |
| BioSpa8 Automated Incubator | BioTek | N/A |
| ImageQuant LAS4000 | GE | N/A |
| Leica White Light Laser Confocal Microscope TCS SP8 | Leica Microsystems | N/A |
| Superdex 200 Increase 10/300 GL | GE Healthcare | Cat# 28990944 |
| HiTrap 5 ml Heparin HP | GE Healthcare | Cat# 17040701 |
| AKTA FPLC System | GE Healthcare | N/A |
| Deposited Data | ||
| Raw data | This paper, Zenodo (10.5281/zenodo.3576389) | https://doi.org/10.5281/zenodo.3576389 |
| Oligonucleotides | ||
| Primers used for plasmid construction | This study, IDT | Table S2 |
| Primers used for construction of EMSA probe | This study, IDT | Table S2 |
| dsDNA fragments (gBlocks) | This study, IDT | Table S2 |
| Recombinant DNA | ||
| Reporter and cI expression constructs | This study | Table S3 |
| cIVP882R11x-HALO-HIS mutants | This study | Table S3 |
| SNAP-Qtip mutants and Ant constructs | This study | Table S3 |
| Software and Algorithms | ||
| Prism v6 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| FIJI v1.52p | FIJI | http://fiji.sc/ |
| LASX v3.5.5.19976 | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| RStudio Desktop 1.2.5033 | RStudio / The R Foundation | https://rstudio.com |