Fig. 5.

Effect of testosterone and Bafilomycin A1 on mitophagy receptor autophagy in m.11778G > A and control lymphoblasts. Cells were grown in standard medium for 4 h with 100 nM testosterone (T), for autophagy inhibition 500 nM Bafilomycin A1 used. a. Relative level of LC3 I and II isoform. b. Lower autophagy flux in m.11778G > A and control lymphoblasts. c. Western-blot analysis for p62, COX IV, β actin served as loading control. Protein immunoreactivity within each blot was normalized to untreated control sample, then the data was combined from 3 independent different blots. Data represented as the mean value ± SD where each experiment was repeated 3 times. For data shown in panels student t test was used: **p < 0.001