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. 2020 Apr 10;10:6201. doi: 10.1038/s41598-020-63052-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE analysis of the non-reduced and reduced states of HEK293 and N. benthamiana (ΔXTFT)-produced CAP256-VRC26 bNAb. M, Protein Ladder; Lane 1, Non-Reduced HEK293-produced CAP256-VRC26.08; Lane 2, Non-Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.08 without hTPST1 coexpression; Lane 3, Non-Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.08 with hTPST1 coexpression; Lane 4, Reduced HEK293-produced CAP256-VRC26.08; Lane 5, Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.08 without hTPST1 coexpression; Lane 6, Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.08 with hTPST1 coexpression; M, Protein Ladder; Lane 7, Non-Reduced HEK293-produced CAP256-VRC26.09; Lane 8, Non-Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.09 without hTPST1 coexpression; Lane 9, Non-Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.09 with hTPST1 coexpression; Lane 10, Reduced HEK293-produced CAP256-VRC26.09; Lane 11, Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.09 without hTPST1 coexpression; Lane 12, Reduced N. benthamiana (ΔXTFT)-produced CAP256-VRC26.09 with hTPST1 coexpression.