Table 2.

Tyrosine sulfated species abundance within CAP256-VRC26 bNAbs as deduced by Intact LC-MS. Mono- and Di-sulfated species percentage of the CAP256-VRC26 bNAbs were derived from the deconvoluted mass spectra of the respective HEK293 and N.

Monoclonal antibody	Non-sulfated Tyr species (%)	Mono-sulfated Tyr species (%)	Di-sulfated Tyr species (%)
HEK293 produced CAP256-VRC26.08	9.88	38.01	52.11
HEK293 produced CAP256-VRC26.09	11.7	52.75	35.55
N. benthamiana produced CAP256-VRC26.08	39.93	39.43	20.64
N. benthamiana produced CAP256-VRC26.09	36.19	36.4	27.41

benthamiana (ΔXTFT)-produced CAP256-VRC26 bNAb (Supplementary Fig. S9).