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. 2020 Apr 10;10:6232. doi: 10.1038/s41598-020-63008-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NO-induced biofilm dispersal. (A) Confocal laser scanning microscopy images used for phenotypic analysis of 72 hrs mature biofilms before and after 2 hrs treatment with 250 μM S150 for PAO1 WT and the deletion mutants ΔrbdA, Δpa2072, ΔfimX and ΔdipA (scale bar 50 μm). (B) COMSTAT analysis of biomass reduction after S150 treatment, as normalised against WT, highlighting differences in biomass reduction for the gene deletion mutants. The Welch’s T-test is used to determine significances, where ***denotes a confidence level of p < 0.01. Data acquired from 3 independent experiments.