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. 2020 Apr 10;10:6232. doi: 10.1038/s41598-020-63008-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Levels of c-di-GMP in planktonic culture, as quantified using a GFP reporter. (A) Relative fluorescence units, which are proportional to intracellular c-di-GMP concentration, normalised to PAO1 WT. (B) The NO-induced decrease in relative fluorescence, and thus relative c-di-GMP concentration, given in percentages. The Student’s T-test was used to determine significances, where ***denotes a confidence level of p < 0.01. Data acquired from 3 independent experiments.