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. 2020 Apr 10;10:6232. doi: 10.1038/s41598-020-63008-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Swimming and twitching phenotypes for the investigated deletion mutants. Swimming agar (A) and twitching agar (C) for the deletion mutants ΔrbdA, Δpa2072, ΔfimX and ΔdipA compared with PAO1 WT, the flagellum mutant ΔfliM, the pili mutant ΔpilA, and the pili flagellum double mutant ΔpilAΔfliM. Scale bar = 1 cm. Data acquired from 6 independent experiments, shown in (B,D), for swimming and twitching areas, respectively, normalised to PAO1 WT. The Welch’s T-test was used to determine significances, where ***denotes a confidence level of p < 0.01.