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. 2020 Apr 10;10:6232. doi: 10.1038/s41598-020-63008-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Swarming phenotypes of the examined mutants in the absence and presence of NO. Swarming motilities were determined for the deletion mutants ΔrbdA, Δpa2072, ΔfimX and ΔdipA and compared with PAO1 WT, the flagellum mutant ΔfliM, the pili mutant ΔpilA, and the pili flagellum double mutant ΔpilAΔfliM. (A) Swarming agar plates, with scale bar = 1 cm. (B) Measured swarming area for each mutant, in the absence of NO, normalised to PAO1 WT. (C) The measured increase in swarming areas on NO donor agar (1 μM SNP), normalised to PAO1 WT, compared to swarming areas on agar without an NO donor. The Welch’s T-test was used to determine significances, where ***denotes a confidence level of p < 0.01. Data acquired from 6 independent experiments.