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Oligonucleotide primers used for cloning and the respective restriction enzyme used. Enzyme restriction sites are underlined.
| Primer Name | Oligonucleotide sequence (5′→3′) | Restriction site |
|---|---|---|
| pET_GFP-AMI.fw | CCGCCGCATATGGAATTCAAAAAAGAAACAGCTAAGAAAAGTGCAAGT | EcoRI |
| pET_GFP-AMI.rv | CCGCCGCTCGAGTCATACAGTAGAACTTGAAGTTCCATTACT | XhoI |
| pET_GFP-SH3.fw | CCGCCGCATATGGAATTCAGACCATCACAAGCAATAATGAATAAATTAAA | EcoRI |
| pET_GFP-SH3.rv | CCGCCGCTCGAGTTAACCTTTGAATACACCCCAGG | XhoI |
