Table 2.

Primers used in multiplex PCR to detect the mcr-1 to mcr-5 genes.

Primer/probe	Amplified gene	Product size (bp)
mcr1_320bp_fw (AGTCCGTTTGTTCTTGTGGC),	mcr-1	mcr-1(320 bp)
mcr1_320bp_rev (AGATCCTTGGTCTCGGCTTG),
mcr2_700bp_fw (CAAGTGTGTTGGTCGCAGTT),	mcr-2	mcr-2 (715 bp)
mcr2_700bp_rev (TCTAGCCCGACAAGCATACC),
mcr3_ 900bp_ fw (AAATAAAAATTGTTCCGCTTATG),	mcr-3	mcr-3 (929 bp)
mcr3_900bp_ rev (AATGGAGATCCCCGTTTTT)
mcr4_1100bp_fw (TCACTTTCATCACTGCGTTG)	mcr-4	mcr-4 (1116 bp)
mcr4_1100bp_rev (TTGGTCCATGACTACCAATG)
mcr5_fw (ATGCGGTTGTCTGCATTTATC)mcr5_fw (ATGCGGTTGTCTGCATTTATC)	mcr-5	mcr-5 (1,644 bp)
mcr5_rev (TCATTGTGGTTGTCCTTTTCTG)