Table 2.
Immune related proteins significantly modulated in the hemolymph of D. polymorpha under both C. parvum and RNA poly I:C challenge. The BLAST annotation and expect value are derived from the sequence similarity search against NCBInr database. H = hemocytes and P = plasma and the fold change of significantly modulated proteins (p-value < 0.05) between treated and control sample is indicated.
| BLAST annotation (NCBInr) | C. parvum | RNA poly I:C | ||||
|---|---|---|---|---|---|---|
| Immune proteins | E-value | Conserved domain | H | P | H | P |
| Pattern recognition receptors (PRRs) | ||||||
| natterin-like protein | 2.5E-09 | jacalin-like lectin domain, C-terminal toxin domain | ‒1.7 | — | — | 1.3 |
| Cation-independent mannose-6-phosphate receptor (M6PR) | 0.0E + 00 | M6PR repeat domains | — | 1.6 | — | 2 |
| Galactose binding lectin | 5.7E-19 | Galactose binding lectin domain | 1.5 | ‒1.6 | — | ‒1.9 |
| Beta-1,3-glucan-binding protein | 5.2E-99 | cytolytic factor, Glycosyl hydrolase domain, beta-1,3-glucan recognition protein | — | −1.3 | — | −1.3 |
| Immune signaling | ||||||
| Macrophage migration inhibitory factor (MIF) | 1.2E-40 | MIF domain | 2.5 | — | 2.3 | — |
| Microorganism Internalization | ||||||
| myosin-2 essential light chain-like | 2.9E-58 | EF-hand, calcium binding motif, Ca2 + -binding protein | 1.7 | — | 1.4 | — |
| integrin beta-like protein | 2.8E-140 | Integrin beta chain VWA domain, Integrin plexin domain | −1.4 | — | — | 1.8 |
| integrin alpha-9-like isoform X2 | 1.9E-54 | integrin alpha domain | — | 1.4 | — | 1.7 |
| Immune effectors | ||||||
| Antimicrobial peptides | ||||||
| Histone H2A.V | 1.3E-54 | Core histone domain, C-terminus of histone H2A | 6 | — | 4.6 | — |
| Proteases, protease inhibitors and hydrolases | ||||||
| cysteine proteinase | 9.3E-145 | Cathepsin propeptide inhibitor domain, Peptidase C1A subfamily | 1.4 | 4.4 | 2.5 | 5.4 |
| serine protease-like protein | 7.4E-19 | Trypsin-like serine protease domain | - | 2.9 | - | 3.2 |
| Metalloproteinase- like protein | 2.9E-212 | Zinc-dependent metalloprotease domain | −1.9 | — | −1.6 | — |
| cystatin B | 1.5E-18 | Cystatin domain | 1.5 | 1.4 | 1.4 | — |
| Sucrase-isomaltase | 0.0E + 00 | Glycoside hydrolases domains | — | 1.4 | — | 2.2 |
| Complement system | ||||||
| complement C1q-like protein | 2.3E-03 | C1q domain | — | −1.7 | — | −2.3 |
| complement C1q-like protein | 2.2E-13 | C1q domain | — | 1.4 | — | 1.5 |
| Complement C1q-like protein | 2.3E-03 | C1q domain | — | −1.6 | — | −1.7 |
| complement factor B-like | 2.8E-22 | von Willebrand factor domain; Complement control protein (CCP) | — | −1.8 | — | −1.6 |
| complement C2 | 8.9E-16 | Trypsin-like serine protease, Von Willebrand factor type A (vWA) domain | — | 1.7 | — | 2.2 |
| complement component C3 | 4.0E-24 | Alpha-2-macroglobulin domain | 1.7 | 1.5 | ||
| complement component C3 | 1.5E-08 | Alpha-2-macroglobulin domain | −2.9 | −3.6 | ||
| Other immune-related process | ||||||
| 26S protease regulatory subunit | 7.3E-217 | 26S proteasome regulatory subunit | 1.5 | 1.7 | ||
| Cytochrome c oxidase | 1.2E-15 | Cytochrome c oxidase subunit Va | 4 | 2.7 | ||
| ubiquitin-conjugating enzyme E2 | 3.3E-72 | catalytic domain of Ubiquitin-conjugating enzyme E2 | 2.5 | 1.9 | ||
| laccase-like protein | 3.2E-43 | — | — | −1.5 | −2.4 | |