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. 2020 Apr 10;10:6243. doi: 10.1038/s41598-020-63227-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chocolate for breakfast imposes a day-night pattern of c-Fos activation in the SCN of rats exposed to the experimental model of shift-work. (A) Representative SCN sections of brains obtained from control (CNT), shift-work (WRK), shift-work + chocolate for dinner (WCH-D) and shift-work + chocolate for breakfast (WCH-B) rats at two time-points, one hour after light onset (empty bars = ZT1) and one hour after lights off (filled bars = ZT13). (B) Number of c-Fos positive cells in the dorsomedial SCN and (C) in the ventrolateral SCN at ZT1 and at ZT13. Data are expressed as the mean + sem (N = 4–8/group). The Tuckey post hoc test indicated statistical difference * day vs night, and & difference from CNT, P < 0.05.