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. 2020 Apr 10;10:6213. doi: 10.1038/s41598-020-63256-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Expression pattern of the EYA4-HR Wt (a), Glu369Asp (b) and Ser534* (c) in COS7 cells. EYA4-HR protein shows a cytoplasmic and nuclear distribution for the Wt and Glu369Asp mutant but no signal was observed for the mutant Ser534*. Hoechst staining was used to identify the nucleus. (B) IMAGE J graphical section showing the fluorescence intensities plotted against the distances (pixels). EYA4-HR staining (in red) for the Wt and Glu369Asp mutant is detected in both the nucleus and the cytoplasm.