Figure 5.

Variation of the cytokine secretion kinetics after C. albicans challenge. Unstimulated and C. albicans-stimulated leucocytes of sixteen subjects were stained for CD4+, CD8+ membrane-antibody markers and for IFN-γ, TNF-α, IL-17, IL-10 and IL-4 secreted cytokines. For each cytokine, the secretion was calculated as the percentage of cytokine secreting cells among CD4+ and CD8+ T cells. Variation of cytokine secretion for myeloid, CD4+ and CD8+ cells after C. albicans challenge. The frequencies of secreted cytokines for each immune cell population was compared to the frequencies of unstimulated cells on day 0 according to the ANOVA model. The fraction of inter-subject variability (white boxes) and C. albicans challenge variability (black boxes) were calculated and expressed as the total sum-of-squares, n = 16, p ≤ 0.05. The inter-subject variability was quantified within the columns as the sum of squares of differences between each subject and the sixteen subjects mean. The post-challenge variability was quantified as the sum of squares of the differences between the column means and the grand mean. The F ratio was the ratio of two sum of square values. Large F ratio signified higher post-challenge variability than inter-subject variability. The P value was determined from the F ratio and the two values of degrees of freedom. Immune cells frequencies data were analyzed according to UI controls, CI and PI outcomes. *P < 0.05; **P < 0.001; ***P < 0.0001; ****P < 0.00001; ns = not significant.