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. 2020 Apr 10;10:6180. doi: 10.1038/s41598-020-63018-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cold acclimation on superficial scald incidence. (a) Schematic representation of the experimental design. Early harvested fruit were cold acclimated at 8 °C for 1 week (H1-acclim, light blue) and compared to fruit not acclimated (H1) or harvested 1 week later (H2). Fruit were stored in classic cold conditions (blue arrow) and phenotyped for scald incidence after one week of shelf-life at room temperature (orange arrow). (b) Incidence of superficial scald injuries after 4 months cold storage according to acclimation treatment and harvest stage on fruit collected from two different orchards (R06 and R11). Values are binomial proportions and confidence intervals for n = 100 and α = 0.05. (c) Effect of acclimation on relative gene expression in fruit peel samples after one day (1D) and one week (1W). Expression relatively to classic cold storage for HSP17.6CII (MD15G1053800), HSP21 (MD13G1108500), HSP90.1 (MD01G1208700), HSP101 (MD06G1201600), PAL (MD12G1116700), PR5 (MD04G1064200), FAR (MD10G1311000) and PPO (MD10G1299400). Data are mean values ± SD of n = 3 for fruit collected in both orchards R06 and R11. Snowflake image unchanged according to https://commons.wikimedia.org/wiki/File:Emojione_2744.svg, (https://creativecommons.org/licenses/by-sa/4.0/deed.en).