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. 2020 Apr 10;10:6197. doi: 10.1038/s41598-020-63279-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-520f-3p directly targets SOX9 in GC. The alignment of the miR-520f-3p sequence with that of predicted SOX9 binding sites. (B) SOX9 expression was assessed via qRT-PCR in HGC-27 and MKN-45 cells following miR-520f-3p or miR-NC transfection. (C) SOX9 levels were assessed via Western blotting in HGC-27 and MKN-45 cells following miR-520f-3p or miR-NC transfection (D) Dual-luciferase reporter assays were used to reveal a reduction in luminescence upon miR-520f-3p mimic co-transfection with the SOX9-Wt vector, without a comparable effect for the SOX9-mut vector in 293 cells. (E) GEPIA data indicated that SOX9 was increased in GC. (F) Measurement of SOX9 expression via qRT-PCR in 92 paired GC and normal gastric epithelial tissue samples. *P < 0.05; **P < 0.01; ***P < 0.001.