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. 2020 Apr 3;144:81–112. doi: 10.3897/phytokeys.144.46700
1. Plants usually with a THC/CBD ratio ≥7; terpenoid profile usually lacks sesquiterpene alcohols, fresh aroma often pleasant. Plants ≥ 2 m tall in good habitats; branches flexible, diverging from the shoot at a relatively acute angle (<45° from vertical). Fresh leaves medium green in color; central leaflets narrow (length/width usually >6), lanceolate to linear-lanceolate; margins with fine to coarse serrations, sometimes biserrate. Mature female inflorescence somewhat compact (flowering stems producing small to medium “buds”), with relatively obscure sugar leaves (a high perigonal bract-to-leaf index); sugar leaves with capitate-stalked glandular trichomes (CSGTs) usually limited to the proximal half of the leaves; perigonal bracts express a moderate to high density of CSGTs. Mature achene exocarp color (beneath the perianth) often green-brown.
A THC/CBD ratio always ≥7, often much more. Mature achenes usually ≥ 3.6 mm long (Fig. 3e, f); perianth mostly sloughed off, but often persistent in places (appearing as irregular spots or stripes); exposed exocarp exhibiting prominent venation; lacking a prominent protuberant base; not readily disarticulating from plant var. indica (“Sativa” in the historical sense2)
B THC/CBD ratio usually ≥7, sometimes less. Mature achenes usually <3.6 mm long (Fig. 3g, h); perianth persistent (covering exocarp and its venation), with strong pigmentation in a mottled or striped pattern; with a protuberant base; readily disarticulating from plant var. himalayensis
2. Plants with a THC/CBD ratio <7; terpenoid profile includes sesquiterpene alcohols, fresh aroma often acrid or “skunky.” Plants < 2 m tall in good habitats, and often ca. 1 m; branches not flexible, branching sometimes nearly 90° from the stalk axis, producing a menorah-shaped habitus. Fresh leaves dark green in color, leaflets of larger leaves sometimes overlap; central leaflets broad (length/width usually <6), often oblanceolate; margins with coarse serrations, rarely biserrate. Mature female inflorescence compact (flowering stems producing medium to large “buds”) with prominent sugar leaves (a low perigonal bract-to-leaf index); sugar leaves have CSGTs extending more than half way down their length; perigonal bracts densely covered with CSGTs. Mature achene exocarp color (beneath the perianth) often a lighter shade of olive green to gray.
A THC/CBD ratio <7 (almost always >2). Mature achenes usually ≥ 3.6 mm long (Fig. 3a, b); perianth mostly sloughed off (appearing as irregular spots or stripes); exposed exocarp exhibiting prominent venation; lacking a prominent protuberant base; not disarticulating from plant, and often trapped in the dense inflorescence var. afghanica (“Indica” in the historical sense2)
B THC/CBD ratio often <2. Mature achenes usually < 3.6 mm long (Fig. 3c, d); perianth persistent (covering exocarp and its venation), with strong pigmentation in a mottled or striped pattern; with a protuberant base; readily disarticulating from plant var. asperrima
1 As emphasized in the text, the differences presented here represent unhybridized plants, before extensive recent hybridization between them.
2 Historically, as discussed in the text, “Sativa” formerly represented landraces of South Asian heritage, and “Indica” formerly represented Central Asian landraces. This key is not intended for the identification of “Sativa” and “Indica” strains commercially available today.